The independent variable in this experiment is the strawberry mixture while the dependent variable is the amount of dna. Each cell in a strawberry contains 8 copies of its genetic information. Strawberry dna extraction lesson plan primary learning. This simple method allows you to extract dna from a strawberry and view it. This is a worksheet that gives the method for the extraction of dna from fruit. Dna, short for deoxyribonucleic acid, is present in all living cells and is the blueprint that tells each cell how to func on.
Dna is the representation of each and every living organism. Add 2 volumes ice cold isopropyl alcohol to the strawberry liquid in the tube. Extracting dna from strawberries dna extraction is an important. Use a funnel and coffee filters to filter the strawberry juice into a beaker. Eighth grade lesson strawberry dna extraction betterlesson. When youve got everyone and their strawberries ready, begin the lab. Make sure juice contains raw, uncooked fruit juice. Humans have two copies of dna in each cell except gametes and red. Strawberries are an exceptional fruit to use for this lesson because each individual student is able to complete the process by themselves and strawberries yield more dna than any other fruit i. Lab procedures should be conducted as stated in the dna extraction. Students will infer which detergent works best to extract dna from the cells.
I predict that dna can be extracted and isolated from strawberry cells by using a dna extraction buffer and alcohol. Experiment sheet for the extraction of dna teaching. This lab was conducted in order to show and analyze the way dna is extracted. For the reason that you want to deliver everything required within a genuine and dependable supply, most people offer valuable home elevators many subjects as well as topics. I chose strawberries because they are easy to manipulate and they contain a large genome. This activity can be easily completed during a 1 hour lesson. I used the alcohol extraction method to isolate dna and to make it visible to the naked eye. Strawberry dna extraction noble research institute. This is a great extension if youve taught cells or the cell theory already. Extract dna from a strawberry national human genome research. Strawberries have an octoploid genome, which means that the nucleus contains. The process of extracting dna from a cell is the first step for many laboratory procedures in biotechnology. Strawberry dna can you extract dna from a strawberry. Once these membranes are broken apart, the dna is released from the cell.
Ethanol or isopropyl alcohol causes the dna to precipitate. Strawberry dna extraction institute for molecular bioscience. Fer dna strawberry extraction lab report mischief canti mean dna strawberry extraction lab report exjew. This is a digital download, of the classic strawberry dna extraction lab. This lab is the most awesome strawberry dna extraction lab because it always works and can be completed with everyday paper products and cleaned up without a lot of fuss. Extracting dna from strawberries book pdf free download link book now. A teaspoons of the dna extraction liquid into the bag with the strawberries. A qualitative comparison of the yield of dna from this lab may be compared to.
Strawberry dna extraction lesson plan this lesson plan is for the. Pour the contents of the 8 oz cup into the tube, cap the tube, wait for the dna to start precipitating out in the alcohol the process begins almost immediately and the dna. The native wild or wood strawberry, fragaria vesca, has only two sets of chromosomes diploid. Dna extraction c 21 chemistry in the k8 classroom grades 48 2007, omsi orpapaya or pineapple juice. In the first part of the lab, we prepared a dna extraction buffer by mixing liquid dish detergent, salt, and distilled. The salt enables the dna strands to come together, or aggregate. Wear safety glasses, lab coat and gloves when performing the experi ment. Strawberries are an exceptional fruit to use for this lesson because each individual student is able to complete the process by themselves and strawberries yield more dna. Deoxyribonucleic acid dna is a molecule that encodes genetic instructions. Dna can be extracted from cells by a simple technique with household chemicals, enabling students to. Use 1 cup of fresh, frozen diluted as directed, or canned juice. Strawberry dna extraction all living things have dna.
It can easily be seen with the naked eye when collected from thousands of cells. Strawberry dna extraction lab worksheet answers by using advantageous issues. Students can enter a competition to see who spools the most dna on a coffee stirrer. For dna extraction, detergent is used to lyse the cell so that dna is released into the solution. Dna extraction the concentrations of the extracted dna was measured using the nanophotometer,uvvis spectrophotometer implen, munich, germany and the average concentration for 12 samples was 0. Dna extraction lesson summary in this lesson, students will remove dna from plant cells strawberries and compare the process to dna extraction in an actual biotech lab will discuss in lecture. Human cells are generally diploid, meaning they have two sets of chromosomes. Many people assume that because dna is so small, we cant see it without powerful microscopes.
Read online strawberry dna extraction lab utah state university book pdf free download link book now. Dna extraction lab for strawberries step by step guide. What is the purpose of the salt solution in this experiment. Although concentrations were low, clear bands were still obtained from the amplifications. I use this sheet to teach pupils to follow instructions and become more independent. Add 10 ml of the dna extraction buffer and mash the strawberry and buffer for about one minute. Strawberry dna extraction background in this activity, you will extract a sample of dna from strawberries. Docx, cheek cell in every cell dna extraction of dna from strawberries. Follow the lab report guidelines laid out in the page. Strawberries are a great choice for a dna extraction lab because they are octoploid, meaning that they have eight copies of each type of dna chromosome.
Strawberry dna extraction activity dna is the amazing and unique chemical instructions that make all living things, from humans to strawberries. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Dna isolation from strawberries uw genome sciences university. Strawberry dna extraction laguardia community college. Strawberries are an easy subject to use since they are readily obtained and processed and because they have multiple copies of their dna in each cell. Download strawberry dna extraction lab utah state university book pdf free download link or read online here in pdf. The long, thick fibers of dna store the information for the functioning of the chemistry of life.
All books are in clear copy here, and all files are secure so dont worry about it. Use noniodized table salt or laboratory grade sodium chloride. Give to students as close to the start of the activity as possible. This is specifically helpful for students being homeschooled if an educator wants to do the same lab but differentiated worksheets for each students age. Materials should not be removed from the classroom. Deoxyribonucleic acid dna is a long molecule that contains the genetic instructions used in the development and functioning of all known living organisms, including the plants we eat. Just before dealing with free printable money worksheets for kindergarten, please are aware that education is usually the factor to a. This is background information for the teacher to assist in facilitating learning and will be explained to the students after the explore section. Strawberries are chosen for this particular experiment because strawberry. Well use strawberries because their cells are easy to break open. Each component of the extraction mixture plays a part. Steps i will explain the purpose of each step in the extraction procedure as it relates to the physical and biochemical characteristics of cells.
Students extracted dna from strawberries and looked at the dna under a microscope with and without a stain dye. The purpose of this experiment is to extract dna from strawberries. Try extracting dna from fruits like bananas or strawberries. Dna strawberry extraction lab report college homework. The purpose of this lab was to extract dna from a strawberry. Dna extraction is important to the study of heredity and to the treatment of many diseases through the creation of gene therapy dna molecules. Dna lab objectives i will extract dna from the fruits that we eat such as strawberry by following dna extraction protocol. These videos represent the students favorite part of the lab and are two steps that i generally warn them about. Strawberry dna extraction lab research paper 667 words. Dna is a double stranded macromolecule composed of nucleotide bases pairing adenine with thymine and guanine with cytosine.
This site is like a library, you could find million book here by using search box in the header. Strawberry dna extraction lab utah state university. This experiment show that dna can be extracted in many different ways from the simplest to the most complex way of extracting it. Strawberry dna extraction lesson plan this lesson plan is for the extraction of dna from strawberries. Related posts of strawberry dna extraction lab worksheet answers free printable money worksheets for kindergarten. Within a few seconds, watch for the development of a white cloudy substance dna in the top layer above the n plastic cup, make your dna extraction liquid. If youre using my lab sheet, i like to begin by reading the included page on the history of dna and the reasons for each step of the extraction process.
Then alcohol is added to cause the dna to precipitate out. The dna precipitates out of the solution when the alcohol is added. The dna found in strawberry cells can be extracted using common, everyday materials. Whichever you use, make sure it is ice cold by placing in an icewater bath or in the freezer. During the dna extraction, the soap pulls apart the fats lipids and proteins that make up the membranes surrounding the cell and nucleus. Back to strawberry dna extraction lab worksheet answers.
Through this experiment we conclude that each and every. The strawberry dna extraction activity, lab protocol and student worksheets were modified and used with permission from julie townsend, parkview middle school, ankeny, ia. In this lab, you will extract strands of dna from the nuclei of strawberry cells. Dna is important because it is the chemical code that instructs each cells activity. How do these steps differ for extraction of dna from bacteria. Dna is present in every cell of plants and animals. Any electrical appliances used in the lab should be. Students will recognize via investigation that all living things have dna within the nucleus of their cells. Extracted dna can also be used to create dna fingerprints to help diagnose genetic diseases, solve criminal cases, identify victims of disaster and war, and establish paternity or maternity. This lesson plan is for the extraction of dna from strawberries.
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